Eannatto Delta Gold > Tocotrienol supplementation suppressed bone resorption and oxidative stress in postmenopausal osteopenic women
Tocotrienol supplementation suppressed bone resorption and oxidative stress in postmenopausal osteopenic women
Summary Tocotrienols have shown bone-protective effect in animals. This study showed that a 12-week tocotrienol supplementation decreased concentrations of bone resorption biomarker and bone remodeling regulators via suppressing oxidative stress in
postmenopausal osteopenic women.
Tocotrienols (TT) have been shown to benefit bone health in ovariectomized animals, a model of postmenopausal
women. The purpose of this study was to evaluate the effect of 12-week TT supplementation on bone markers (serum bonespecific alkaline phosphatase (BALP), urine N-terminal telopeptide (NTX), serum soluble receptor activator of nuclear factorkappaB ligand (sRANKL), and serum osteoprotegerin (OPG)), urine calcium, and an oxidative stress biomarker (8-hydroxy-2′-
deoxyguanosine (8-OHdG)) in postmenopausal women with osteopenia.
Eighty-nine postmenopausal osteopenic women (59.7 ± 6.8 year, BMI 28.7 ± 5.7 kg/m2
) were randomly assigned to
three groups: (1) placebo (430 mg olive oil/day), (2) low TT (430 mg TT/day, 70% purity), and (3) high TT (860 mg TT/day, 70%
purity). TT, an extract from annatto seed with 70% purity, consisted of 90% delta-TT and 10% gamma-TT. Overnight fasting
blood and urine samples were collected at baseline, 6, and 12 weeks for biomarker analyses. Eighty-seven subjects completed the
Relative to the placebo group, there were marginal decreases in serum BALP level in the TT-supplemented groups over
the 12-week study period. Significant decreases in urine NTX levels, serum sRANKL, sRANKL/OPG ratio, and urine 8-OHdG
concentrations and a significant increase in BALP/NTX ratio due to TT supplementation were observed. TT supplementation did
not affect serum OPG concentrations or urine calcium levels throughout the study period. There were no significant differences in
NTX level, BALP/NTX ratio, sRANKL level, and sRANKL/OPG ratio between low TT and high TT groups.
Twelve-week annatto-extracted TT supplementation decreased bone resorption and improved bone turnover rate via
suppressing bone remodeling regulators in postmenopausal women with osteopenia. Such osteoprotective TT’s effects may be, in
part, mediated by an inhibition of oxidative stress.