Eannatto Delta Gold > Exploring the potential of tocotrienol from Bixa orellana as a single agent targeting metabolic syndrome and bone loss
Exploring the potential of tocotrienol from Bixa orellana as a single agent targeting metabolic syndrome and bone loss
Metabolic syndrome (MetS) is associated with osteoporosis due to the underlying inflammatory and hormonal
changes. Annatto tocotrienol has been shown to improve medical complications associated with MetS or bone
loss in animal studies. This study aimed to investigate the effects of annatto tocotrienol as a single treatment for
MetS and osteoporosis in high-carbohydrate high-fat (HCHF) diet-induced MetS animals. Three-month-old male
Wistar rats were randomly divided into five groups. The baseline group was euthanized at the onset of the study.
The normal group received standard rat chow and tap water. The remaining groups received HCHF diet and
treated with three different regimens orally daily: (a) tocopherol-stripped corn oil (the vehicle of tocotrienol), (b)
60 mg/kg annatto tocotrienol, and (c) 100 mg/kg annatto tocotrienol. At the end of the study, measurements of
MetS parameters, body compositions, and bone mineral density were performed in animals before sacrifice.
Upon euthanasia, blood and femur of the rats were harvested for the evaluations of bone microstructure, biomechanical strength, remodelling activities, hormonal changes, and inflammatory response. Treatment with
annatto tocotrienol improved all MetS parameters (except abdominal obesity), trabecular bone microstructure,
bone strength, increased osteoclast number, normalized hormonal changes and inflammatory response in the
HCHF animals. In conclusion, annatto tocotrienol is a potential agent for managing MetS and osteoporosis
concurrently. The beneficial effects of annatto tocotrienol may be attributed to its ability to prevent the hormonal changes and pro-inflammatory state in animals with MetS